HISEQ 2500 & MISEQ:

ION TORRENT PERSONAL GENOME MACHINE:

Illumina HiSeq 2500 and MiSeq - provides high sequencing output at relatively low cost, combining speed with the ability to sequence across a wide range of applications Life Technologies Ion Torrent personal genome machine – suitable for small genome sequencing
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WHICH PLATFORMS FOR WHICH APPLICATIONS?

PLATFORM: Illumina HiSeq Illumina MiSeq Life Technologies
Ion Torrent PGM
POPULAR APPLICATIONS
  • Transcriptomics and RNA-seq
  • Whole genome sequencing
  • De novo sequencing
  • Human exome sequencing
  • Metagenomics and amplicon sequencing
  • Bacterial whole genome sequencing
  • Small RNA sequencing
  • Targeted sequencing of selected genes: custom and community amplicon panels (AmpliSeq) are also available
  • Whole genome sequencing of small genomes
OUTPUTS Up to 56 Gb* per lane, with read lengths ranging from 1 x 50 bp SE to 2 x 250bp PE Up to 12 Gb** per run, with read lengths ranging from 2 x 25 PE to 2 x 300 bp PE Scalable from 10Mb to 1Gb per run, with read lengths of 200 or 400 bases
NUMBER OF READS Up to 225 million paired-end reads per lane Up to 45 million paired-end reads per run >6 million per run (318 chip)

* Output based on 2 x 125 bp PE run
** Output is based on a 2 x 300 bp PE run

SEQUENCING APPLICATIONS

WHOLE GENOME SEQUENCING:

A process that aims to determine the complete DNA sequence of an organism.

De Novo SEQUENCING:

Assembling a previously-uncharacterised de novo genome or a re-sequenced genome is relevant to SNP discovery, insertions, deletions and large-scale rearrangements.

TRANSCRIPTOMICS:

Enable the study of cellular RNAs to improve existing gene annotations and discover new transcripts and splice variants. RNA sequencing can also provide a comparative analysis of the transcriptome between multiple samples. Multiplexing options are available.

METAGENOMICS AND AMPLICON SEQUENCING:

Metagenomics enables the study of microorganisms in their natural environment, by whole genome sequencing or targeted approaches (such as 16S or 18S sequence interrogation). Combining amplicon sequencing with indexing techniques enables large numbers of samples to be sequenced at low costs.

HUMAN EXOME SEQUENCING:

A cost-effective solution to discover common and rare variants for a range of human diseases, targeted capture (1-2% of the total genome) and re-sequencing of the human exome. DNA from multiple individuals can be indexed and captured using one array.

CUSTOM PROJECTS:

If your project requires an application not specified above, call or email via the project enquiry form and we will contact you to discuss your needs.

A COLLABORATION OF:

WITH THE SUPPORT OF:

Ministry of Business, Innovation & Employment